Preprints under consideration

Bahnan, W. et al. Protection induced by a human monoclonal antibody recognizing two different epitopes in a conserved region of streptococcal M proteins. BioRxiv (2021). doi:10.1101/2021.03.01.433494
Kumra Ahnlide, V., Kumra Ahnlide, J., Beech, J. P. & Nordenfelt, P. Binding site localization on non-homogeneous cell surfaces using topological image averaging. BioRxiv (2020). doi:10.1101/2020.11.03.366476



Kumra Ahnlide V, de Neergaard T, Sundwall M, Ambjörnsson T, Nordenfelt P. A Predictive Model of Antibody Binding in the Presence of IgG-Interacting Bacterial Surface Proteins. Front Immunol (2021) 12
de Neergaard T, Sundwall M, Wrighton S, Nordenfelt P. High-Sensitivity Assessment of Phagocytosis by Persistent Association-Based Normalization. J Immunol (2021) 206:214–224.


Du Rietz, H., Hedlund, H., Wilhelmson, S., Nordenfelt, P. & Wittrup, A. Imaging small molecule-induced endosomal escape of siRNA. Nat Commun (2020).


Mohanty, T., Nordenfelt, P., Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT. J. Vis. Exp. 2019. Nov 27 (153).
Happonen, L., Hauri, S., Svensson Birkedal, G., Karlsson, C., de Neergaard, T., Khakzad, H., Nordenfelt, P., Wikström, M., Wisniewska, M., Björck, L., Malmström, L., Malmström, J., 2019. A quantitative Streptococcus pyogenes-human protein-protein interaction map reveals localization of opsonizing antibodies. Nature Communications. 10, 2727.


Nordenfelt, P., Cooper, J., and Hochstetter, A., “Matrix-masking to balance nonuniform illumination in microscopy,” Opt. Express 26, 17279-17288

Mohanty, T., Sørensen, O., and Nordenfelt, P. NETQUANT: automated quantification of neutrophil extracellular traps. Frontiers In Immunology 8, 1999.


Nordenfelt, P., Moore, T.I., Mehta, S.B., Kalappurakkal, J.M., Swaminathan, V., Koga, N., Lambert, T.J., Baker, D., Waters, J.C., Oldenbourg, R., Tani, T., Mayor, S., Waterman, C.M. and Springer, T.A. Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration. Nature Communications, 2017; 8 (1), p. 2047.
Media coverage:
TV broadcast in Vetenskapens Värld, SVT. Celler rör sig med kraft inifrån.
Interview at Lund University. “Så gör dina celler för att svänga.
Reported in myScience. “How cells are able to turn.
Reported in News Medical. “Researchers explore how cells navigate the body.

Swaminathan V, Kalappurakkal JM, Mehta SB, Nordenfelt P, Moore TI, Koga N, Baker D, Oldenbourg R, Tani, T, Mayor S, Springer TA, Waterman CM. Actin retrograde flow actively aligns and orients ligand-engaged integrins in focal adhesions. Proceedings of the National Academy of Sciences. 2017 Oct 3;114(40):10648–53.
Media coverage:
– Reported by Marine Biological Laboratory, Woods Hole. “Internal Forces Directing Cell Migration are Revealed by Live-Cell Microscopy.

Danielsen J, Nordenfelt P. Computer Vision-Based Image Analysis of Bacteria.
Methods Mol Biol. 2017;1535:161-172. PMID: 27914078

Shannon O, Nordenfelt P. Measuring Antibody Orientation at the Bacterial Surface.
Methods Mol Biol. 2017;1535:331-337. PMID: 279140902016

Nordenfelt P., Collin M. Bacterial pathogenesis: methods and protocols. Methods Mol Biol. 2017. 1535.


Nordenfelt, P., Elliott, H.L., and Springer, T.A. Coordinated integrin activation by actin-dependent force during T cell migration. Nature Communications. 2016. Oct 10;7:13119. PMID:27721490. Link to pdf

Media coverage:
Interview at Lund University. “How cells move.
Reported in Science Daily. “How cells move.


Lood, R., Wollein Woldetoft, K., and Nordenfelt P. Localization-triggered bacterial pathogenesis. Future Microbiology. 2015. Oct;10:1659-68. PMID: 26437846.
– Made the cover! 

Hurley, S., Kahn, F., Nordenfelt, P., Mörgelin M., Sørensen O.E., and Shannon, O. Platelet-dependent neutrophil function is dysregulated by M protein from Streptococcus pyogenesInfection and immunity. 2015; 83(9), pp.3515–3525. PMID: 26099589


Malmström, E., Davidova, A., Mörgelin, M., Linder, A., Larsen, M., Qvortrup, K., Nordenfelt, P., Shannon, O., Dzupova, O., Holub, M., Malmsström, J., and Herwald, H. Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression. Thrombosis and Haemostasis. 2014; 112(6), 1230–1243. PMID: 2510441

Nordenfelt, P. Quantitative assessment of neutrophil phagocytosis using flow cytometry. Methods in Molecular Biology. 2014; 1124, 279–289. PMID: 24504959


Nordenfelt, P. and Björck, L. IgG-binding bacterial proteins and pathogenesis. Future Microbiology, 2013; 8, 299–301. PMID: 23464367


Nordenfelt, P., Waldemarson, S., Linder, A., Mörgelin, M., Karlsson, C., Malmström, J., and Björck, L. Antibody orientation at bacterial surfaces is related to invasive infection. Journal of Experimental Medicine, 2012; 209(13), 2367–2381. PMID: 23230002
Media coverage:
– Main cover story!
– Commentary – Nature Reviews Immunology
– Commentary – Nature Reviews Microbiology
Article of the Month – Lund Medical Faculty Monthly
Commentary – Science Daily

Malmström, L., Nordenfelt, P., and Malmström, J. 
Business intelligence strategies enables rapid analysis of quantitative proteomics data. Journal of Proteome Science & Computational Biology. 2012; 1. 2050-2273-1-5

Nordenfelt, P., Grinstein, S., Björck, L., & Tapper, H. V-ATPase-mediated phagosomal acidification is impaired by Streptococcus pyogenes through Mga-regulated surface proteins. Microbes and Infection. 2012; 14(14), 1319-1329. PMID: 22981599

Malmström, J., Karlsson, C., Nordenfelt, P., Ossola, R., Weisser, H., Quandt, A., Hansson, K., Aebersold, R., Malmström, L, and Björck L. Streptococcus pyogenes in Human Plasma: Adaptive mechanisms analyzed by mass spectrometry-based proteomics. Journal of Biological Chemistry. 2012; 287(2), 1415–1425. PMID: 22117078


Nordenfelt, P. and Tapper, H. Phagosome dynamics during phagocytosis by neutrophils. Journal of Leukocyte Biology. 2011; 90(2), 271–284. PMID: 21504950
– Main cover story! 


Källquist, L., Rosén, H., Nordenfelt, P., Calafat, J., Janssen, H., Persson, A.-M., Hansson, M, & Olsson, I. Neutrophil elastase and proteinase 3 trafficking routes in myelomonocytic cells. Experimental Cell Research. 2010; 316(19), 3182–3196. PMID: 20828556

Nordenfelt, P., and Tapper, H. The role of calcium in neutrophil granule-phagosome fusion. Communicative & Integrative Biology, 2010; 3(3), 1–3. PMID: 20714398


Nordenfelt, P., Bauer, S., Lönnbro, P., & Tapper, H. Phagocytosis of Streptococcus pyogenes by all-trans retinoic acid-differentiated HL-60 cells: roles of azurophilic granules and NADPH oxidase. PLoS ONE. 2009; 4(10), e7363. PMID: 19806211

Nordenfelt, P., Winberg, M. E., Lönnbro, P., Rasmusson, B., and Tapper, H. Different requirements for early and late phases of azurophilic granule-phagosome fusion. Traffic. 2009; 10(12), 1881–1893. PMID: 19804565


Lönnbro, P.#, Nordenfelt, P.#, and Tapper, H. Isolation of bacteria-containing phagosomes by magnetic selection. BMC Cell Biology. 2008; 9, 35. PMID: 18588680. # shared first-authorship

Linge, H.M., Collin, M., Nordenfelt, P., Mörgelin, M., Malmsten, M., Egesten, A. The human CXC chemokine granulocyte chemotactic protein 2 (GCP-2)/CXCL6 possesses membrane-disrupting properties and is antibacterial. Antimicrobial Agents and Chemotherapy. 2008 Jul;52(7):2599-607. PMID: 18443119


Lönnbro, P., Nordenfelt, P., and Tapper, H. Analysis of neutrophil membrane traffic during phagocytosis. Methods in Molecular Biology. 2007; 412, 301–318. PMID: 18453120

Protection induced by a human monoclonal antibody recognizing two different epitopes in a conserved region of streptococcal M proteins